| IGFBP-3

Insulin-like growth factor-binding protein 3, also known as IGFBP-3, is a protein encoded by the IGFBP3 gene. IGFBP-3 was first isolated, characterized, and quantitated in human plasma, in 1986. It has well-documented functions in the circulation, in the extracellular environment, and inside cells. It is the main IGF transport protein in the bloodstream, where it carries the growth factors predominantly in stable complexes that contain the binding protein, either IGF-1 or IGF-2, and a third protein called the acid-labile subunit or ALS.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	300	150	75	37.5	18.75	9.37	4.68	0
OD	2.22	1.34	0.75	0.48	0.33	0.25	0.2	0.08
Corrected OD	2.14	1.26	0.67	0.4	0.25	0.17	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse IGFBP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Mouse IGFBP-3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	89-96
	Average (%)	91	94
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98